Position-independent germline transformation inDrosophilausing a cuticle pigmentation gene as a selectable marker
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چکیده
منابع مشابه
Position-independent germline transformation in Drosophila using a cuticle pigmentation gene as a selectable marker.
P element mediated germline transformation is an essential part of the analysis of gene function in Drosophila melanogaster. This method allows for efficient introduction of single copies of genes into random positions within the genome (1). The generation of multiple transformed lines is required, as some genes display variable expression patterns depending on the insertion site (2, 3). Elimin...
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Transgene technology provides a powerful tool for developing traits that are otherwise difficult to achieve through conventional methods. The development of transgenic plants requires the use of selectable marker genes, as the efficiency of plant transformation is less than optimal for many important species, especially for monocots such as durum wheat (Triticum turgidum var. durum). Many conce...
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A positive selectable marker system was adapted for transformation of mature embryo-derived calli of Indica rice (Oryza sativa L.) utilizing the PMI gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. The transformed cells grew on medium supplemented with 3% mannose as carbon source and calli were selected on media containing various concentrati...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.21.5859